β-mercaptoethanol assists efficient construction of sperm bacterial artificial chromosome library
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چکیده
منابع مشابه
Construction and evaluation of a porcine bacterial artificial chromosome library.
A porcine bacterial artificial chromosome (BAC) library consisting of 103,488 clones has been constructed. The average insert size in the BAC vector was calculated to be 133 kb based on the examination of 189 randomly selected clones, indicating that the library contained 4.4 genome equivalents. The library can be screened by two-step PCR. The first screening step is performed on 22 superpools,...
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A genomic bacterial artificial chromosome (BAC) library of the A genome of wheat has been constructed. Triticum monococcum accession DV92 was selected for this purpose because it is a cultivated diploid wheat and one of the parental lines used in the construction of a saturated genetic map. Leaves from this accession were used to isolate high-molecular-weight DNA from nuclei. This DNA was parti...
متن کاملConstruction and preliminary characterization of a river buffalo bacterial artificial chromosome library.
River buffalo genome analyses have advanced significantly in the last decade, and the genome sequence of Bubalus bubalis will be available shortly. Nonetheless, large-insert DNA library resources such as bacterial artificial chromosomes (BAC) are still required for validation and accurate assembly of the genome sequence. We constructed a river buffalo BAC library containing 52,224 clones ...
متن کاملConstruction and characterization of a bacterial artificial chromosome library for the allotetraploid Gossypium tomentosum.
Gossypium tomentosum is a wild allotetraploid species with the (AD)5 genome. It is characterized by many useful traits including finer fiber fineness, drought tolerance, and Fusarium and Verticillium resistance. We constructed the first bacterial artificial chromosome library for Gossypium tomentosum. With high quality and broad coverage, this library includes 200,832 clones, with an average in...
متن کاملConstruction and Characterization of a Bacterial Artificial Chromosome Library for the Hexaploid Wheat Line 92R137
For map-based cloning of genes conferring important traits in the hexaploid wheat line 92R137, a bacterial artificial chromosome (BAC) library, including two sublibraries, was constructed using the genomic DNA of 92R137 digested with restriction enzymes HindIII and BamHI. The BAC library was composed of total 765,696 clones, of which 390,144 were from the HindIII digestion and 375,552 from the ...
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ژورنال
عنوان ژورنال: Journal of Biological Methods
سال: 2017
ISSN: 2326-9901
DOI: 10.14440/jbm.2017.167